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As a result, the immunoglobulin is effectively immobilized on the surface of the azopolymer without chemical modi cation This novel method is useful for the immobilization of a variety of small particles such as charged proteins, negatively charged DNA, and hydrophobic polystyrene microspheres on azopolymer surfaces, and it has been shown that the immobilized biomolecules can maintain their higher order structure without damage to their functionality This versatility in terms of immobilization is a signi cant advantage of this technique The photoinduced immobilization technique is closely related to the formation process used for surface relief gratings (SRG) because both phenomena are based on mass transportation of the azopolymer surface The relief structure on the azopolymer surface is induced by the interference of the two coherent beams that are used for the irradiation, with the same periodic structure as the interference light, as shown in Fig 93.

3

Intensive studies for SRG formation have been reported since it was rst developed in 1995 (Batalla et al, 1995; Kim et al, 1995) Various deformed structures that can be induced by photoirradiation.

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Figure 9.3. Formation of SRG on an azopolymer, as generated by two-beam interference irradiation. The grating pitch, Lgr is determined by the wavelength and the incident angle of the irradiated light. The gure on the right exhibits a topographical image as measured by AFM.

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have been demonstrated in addition to SRG (Fukuda et al., 2001; Ye et al., 2001). The surface deformation mechanism needs to be understood by considering not only photochemical phenomena involving the azobenzene moiety and the mobility of the polymer matrix but also by interactions of the irradiation light with electric elds. Therefore, a large number of researchers are making continued efforts to clarify the complex deformation mechanism (Barada et al., 2006; Barret et al., 1996; Kumar et al., 1998; Pedersen et al., 1998).

And ends with this: 32

The authors have recently switched the objectives of their research into SRG formation to now consider interactions between small objects and azopolymer surfaces, such as mass transportation and molecular reorientation, and have investigated photoinduced nanofabrication using a novel approach They have discovered some interesting phenomena that are applicable to nanometer-scale fabrication by irradiating light onto microobjects set on azopolymer surfaces (Keum et al, 2003; Hasegawa et al, 2002b, 2001; Ikawa et al, 2001a,b, 2000; Watanabe et al, 2006, 2001, 2000) The resolution of recording or fabrication processes that is de ned by light is determined by how narrowly the irradiating light can be focused, and, because of to diffraction limits, in practice this equates to about half of the wavelength of the irradiating light.

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